RecombinantHumanInterleukin-1ReceptorAntagoNIST(rHuIL-1RA)


ORDERINGINFORMATION


Formulation:Sterile-filteredwhitelyophilizedpowderwithnoadditives.Purifiedbyproprietarychromatographictechniques.


BACKGROUND

Interleukin-1RAisamemberoftheInterleukin-1(IL-1)cytokinefamily.ItinhibitstheactivitiesofofIL-1alphaandIL-1betaandmodulatesavarietyofIL-1-relatedimmuneandinflammatoryresponses.Apolymorphismofthisgeneisreportedtobeassociatedwithanincreasedriskofosteoporoticfracturesandgastriccancer.


DESCRIPTION

RecombinanthumanIL-1RAproducedinE.coliisanon-glycosylatedN-terminalmethionylformofthenaturally-occurringpolypeptidecontaining153aminoacidswithamolecularweightof17kDa.

SPECIFICATIONSUMMARY

CatalogNumber

Size

40101P-20

20ug

40101P-100

100ug

40101P-1000

1000ug

CatalogNumber

Size

40101P-20

20ug

40101P-100

100ug

40101P-1000

1000ug

Source:Escherichiacoli

Purity:Greaterthan98%asdeterminedbySDS-PAGEandRP-HPLC.

Accessionnumber:P18510.1

Aminoacidsequence:ThesequenceofthefirstfiveN-terminalaminoacidsisMet-Arg-Pro-Ser-Gly.


BIOLOGICALACTIVITY

ED50bydose-dependentinhibitionofIL-1stimulationofD10Scellsis0.5ng/mlwhichcorrespondstoaSpecificActivityof2,000,000IU/mg.


RECONSTITUTION

ReconstituteinsteriledistilledH2Otonotlessthan100ug/mlwhichcanbefurtherdilutedinotheraqueoussolutions.


STORAGEANDSTABILITY

Storedessicatedbelow-18oC.Afterreconstitution,storeat4oCfor2-7daysandbelow-18oCforfutureuse.Forlongtermstorageofthereconstitutedproduct,additionof0.1%humanserumalbuminorbovineserumalbuminisrecommended.Avoidmultiplefreeze-thawcycles.


Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.